Analysis Note

Positive ControlHeLa cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)

General description

Purified mouse monoclonal antibody. Recognizes the ~140-145 kDa MCM2 protein.

This Anti-MCM2 Mouse mAb (6A8) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of MCM2.

Recognizes the ~140-145 kDa MCM2 protein in HeLa cells.

Immunogen

A recombinant polypeptide corresponding to amino acids of 805-904 human MCM2, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)